FastQCFastQC Report
Wed 28 Aug 2019
H5WYNBGXC_n02_cDNA_BRD4_1d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5WYNBGXC_n02_cDNA_BRD4_1d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18445295
Sequences flagged as poor quality0
Sequence length49
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT1651960.8955996637624932No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG468500.25399431128642835No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT302760.16413941875150276No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT237950.032.593861
ATCAACG248850.030.93972411
AGCAGTG254100.030.5548952
TCAACGC253750.030.28295712
CAACGCA256300.030.0328913
AACGCAG257100.029.93221314
GTATCAA259900.029.6330369
ACGCAGA260400.029.61060715
AGAGTAC258550.029.60601419
TATCAAC261400.029.44614210
GGTATCA267500.028.7173068
TGGTATC268100.028.6450967
CGCAGAG270200.028.53618616
AGTGGTA272900.028.2044415
GTACTTT271050.027.81820322
GCAGAGT277800.027.70868117
CAGTGGT279800.027.692124
CAGAGTA282750.027.23186318
GAGTACT279700.027.22848720
GTGGTAT292900.026.314986
Produced by FastQC (version 0.11.5)