FastQCFastQC Report
Wed 28 Aug 2019
H5WYNBGXC_n02_cDNA_BRD4_1c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5WYNBGXC_n02_cDNA_BRD4_1c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18001014
Sequences flagged as poor quality0
Sequence length49
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT1625350.9029213576524078No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG435330.24183637655078766No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT295550.16418519534510667No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT233000.032.1913381
ATCAACG242550.030.64392911
AGCAGTG248100.030.2466722
TCAACGC247100.030.09673312
CAACGCA249950.029.76241113
AACGCAG251750.029.61037814
GTATCAA253050.029.3983769
ACGCAGA256000.029.08520715
AGAGTAC254550.028.98083119
GGTATCA256750.028.9399348
TATCAAC257750.028.89511110
TGGTATC259250.028.6358227
AGTGGTA266500.027.9458545
CGCAGAG268850.027.78239816
GTACTTT265600.027.40968322
CAGTGGT273550.027.2643934
GCAGAGT275050.027.0000317
GAGTACT272550.026.89322320
CAGAGTA278050.026.70901318
GTGGTAT285450.026.0527526
Produced by FastQC (version 0.11.5)