FastQCFastQC Report
Wed 28 Aug 2019
H5WYNBGXC_n02_cDNA_BRD4_1b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5WYNBGXC_n02_cDNA_BRD4_1b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31048776
Sequences flagged as poor quality0
Sequence length49
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT2551600.8218037322952763No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG639540.20597913424992986No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT479020.1542798337686484No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT379750.031.6392631
ATCAACG391450.030.5110711
AGCAGTG401650.029.983252
TCAACGC399850.029.90745212
CAACGCA404400.029.5770813
AACGCAG409000.029.2452314
GTATCAA410850.029.0286399
ACGCAGA412000.029.01140415
AGAGTAC408300.028.97432319
TATCAAC415950.028.74471310
GGTATCA419450.028.4682468
TGGTATC419250.028.4356257
CGCAGAG431750.027.75353216
AGTGGTA431400.027.7097425
GTACTTT429250.027.29308922
GCAGAGT441450.027.01741617
CAGTGGT447150.026.8195744
GAGTACT443200.026.54701220
CAGAGTA453150.026.3057418
GTGGTAT464400.025.7311136
Produced by FastQC (version 0.11.5)