FastQCFastQC Report
Wed 28 Aug 2019
H5WYNBGXC_n02_cDNA_BRD4_1a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5WYNBGXC_n02_cDNA_BRD4_1a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18158415
Sequences flagged as poor quality0
Sequence length49
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT1652360.909969289720496No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG447130.2462384519794266No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT295790.16289417330752712No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT241400.032.4765431
AGCAGTG251950.031.2936732
ATCAACG251950.030.89104811
TCAACGC257650.030.25763112
CAACGCA260050.029.9792113
AACGCAG262100.029.78664614
GTATCAA263050.029.6129479
ACGCAGA264850.029.44489115
AGAGTAC263600.029.35645719
TATCAAC265550.029.34144810
GGTATCA269850.028.96138
TGGTATC271000.028.806677
CGCAGAG275000.028.41260316
AGTGGTA276600.028.3478975
GTACTTT274050.027.9215222
CAGTGGT281850.027.8650194
GCAGAGT282650.027.55247717
GAGTACT282050.027.32140720
CAGAGTA288850.026.96152518
GTGGTAT292950.026.706976
Produced by FastQC (version 0.11.5)