FastQCFastQC Report
Wed 28 Aug 2019
H5WYNBGXC_n02_HTO_BRD4_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5WYNBGXC_n02_HTO_BRD4_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8893576
Sequences flagged as poor quality0
Sequence length49
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG566620.637111551079116No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAC2814300.042.803661
ACTGTCT1808750.042.800041
TTACTAT2833800.042.742124
TACTATC2831950.042.7401165
GTCTAAC1810250.042.7337074
CGTATTC1135150.042.7329447
CTTACTA2837300.042.7267233
TCTAACG1810100.042.7016265
TATCCCC2821950.042.6916738
GGCTGAG2438350.042.690913
GTATTCC1136350.042.6861658
CTATCCC2824500.042.6726847
CTAACGG1804900.042.6697966
TGTCTAA1813800.042.6510243
TGAGCTA2444900.042.645376
ATCCCCC2806650.042.644189
ACTATCC2835600.042.6441576
ACGTATT1141700.042.6438456
GCTGAGC2439650.042.6337134
CTGAGCT2447600.042.60365