FastQCFastQC Report
Wed 28 Aug 2019
H5WYNBGXC_n02_HTO_BRD4_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5WYNBGXC_n02_HTO_BRD4_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11897623
Sequences flagged as poor quality0
Sequence length49
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG584260.49107288069221894No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCTA2769600.042.8758352
AGCTTAC4347100.042.8600041
CTTACTA4373450.042.846343
TTACTAT4371550.042.836754
GCTTACT4346900.042.8181572
ACTGTCT2773700.042.794581
TGTCTAA2772200.042.7910963
TACTATC4372600.042.78065
GTCTAAC2772550.042.776984
CTAACGG2763400.042.7507556
GAGGCTG2839500.042.745181
TCTAACG2771350.042.739235
TATCCCC4355800.042.699128
ACTATCC4374500.042.692056
CTATCCC4360050.042.6880727
TAACGGC2763950.042.6846857
CGTATTC1097850.042.6746337
ATCCCCC4331550.042.6611949
GGCTGAG2837500.042.6610263
TGAGCTA2841700.042.6479076