FastQCFastQC Report
Wed 28 Aug 2019
H5WYNBGXC_n02_GDO_BRD4_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5WYNBGXC_n02_GDO_BRD4_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18529824
Sequences flagged as poor quality0
Sequence length49
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATAGCTCTTAAACCGGTGTAGATCAGGCGGCCCCCCCATATAAGAAA205439311.086953659138912No Hit
GCATAGCTCTTAAACCGTAGCTGAGAGTTGGTGCTCCCCATATAAGAAA12391146.687133131971463No Hit
GCATAGCTCTTAAACTTGCGACGCTTAGCCTCCGTCCCCATATAAGAAA10025775.4106126426241286No Hit
GCATAGCTCTTAAACAACTTCACTCTTTGGACAGCCCCCATATAAGAAA9205484.9679263008650265No Hit
GCATAGCTCTTAAACGGACTATCATGATCTCGGTTCCCCATATAAGAAA7931874.280596512951229No Hit
GCATAGCTCTTAAACGCGTAAGAATAACAGTGGTCCCCCATATAAGAAA7150313.8588116109467636No Hit
GCATAGCTCTTAAACACACGACTACTGTGACATCACCCCATATAAGAAA6875403.7104507846377817No Hit
GCATAGCTCTTAAACCATTGAATTCGCCTACACGGCCCCATATAAGAAA6527043.522451157658055No Hit
GCATAGCTCTTAAACAGCGCTTCTTTATTGTTCCCCCCCATATAAGAAA5057202.72922182099517No Hit
GCATAGCTCTTAAACCACTGTTGTGGTGGATGCAGCCCCATATAAGAAA4744782.5606179529821764No Hit
GCATAGCTCTTAAACTTGAACGGGCCGCGGAAGCGCCCCATATAAGAAA4634892.5013135580780474No Hit
GCATAGCTCTTAAACGGATATAGTCAACAGGATTCCCCCATATAAGAAA4500122.4285821603054623No Hit
GCATAGCTCTTAAACCTGTTGATTTAGACGGTATGCCCCATATAAGAAA4439712.3959806633889236No Hit
GCATAGCTCTTAAACAGATCTATATCTTGCCTCCACCCCATATAAGAAA4242042.2893039890718874No Hit
GCATAGCTCTTAAACCGCCGTTAAGCGGAAACGATCCCCATATAAGAAA3733702.0149678701751297No Hit
GCATAGCTCTTAAACAATTGTAGACGTTCTCCACACCCCATATAAGAAA3686991.9897598595647752No Hit
GCATAGCTCTTAAACCACTGAATTCTCAGTACTGTCCCCATATAAGAAA3182931.7177335305505332No Hit
GCATAGCTCTTAAACCCGGCAGAAATGTCGAGTGGCCCCATATAAGAAA3084611.6646731237166634No Hit
GCATAGCTCTTAAACTAACGTACAACTTGATCTCGCCCCATATAAGAAA2667891.4397816190806778No Hit
GCATAGCTCTTAAACCCCTGTTGTTGACTTCAACGCCCCATATAAGAAA2344391.2651982015587413No Hit
GCATAGCTCTTAAACCTCGGGAGAAAGAGGAGCGTCCCCATATAAGAAA2235051.2061906254479264No Hit
GCATAGCTCTTAAACCGGTGTAGATCAGGCGGCCCCCCCCATATAAGAA1875731.012276209423252No Hit
GCATAGCTCTTAAACACGTATGAGTCGGAGGAAGACCCCATATAAGAAA1866321.0071979097049166No Hit
GCATAGCTCTTAAACCAGGACAGCAACTTCTGCCCCCCCATATAAGAAA1834430.9899878163980403No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1804080.9736088157124428No Hit
GCATAGCTCTTAAACCCTTTCTGATATCCAGTTTACCCCATATAAGAAA1708560.922059486371808No Hit
GCATAGCTCTTAAACCGGTGTAGATCACGCTGCACCCCCAAAAAATAAA857990.46303192086443995No Hit
GCATAGCTCTTAAACCCCAGTTCATGGCCCACAAGCCCCATATAAGAAA757320.40870328827731983No Hit
GCATAGCTCTTAAACACGTATATCCCGAATTAATGCCCCATATAAGAAA720070.3886005609119655No Hit
GCATAGCTCTTAAACCCGCGCCGCTCGGGAGAAAGCCCCATATAAGAAA612290.3304348708330959No Hit
GCATAGCTCTTAAACCGTAGCTGAGAGTTGGTGCTCCCCCATATAAGAA387810.2092896295183376No Hit
GCATAGCTCTTAAACCAGGACAGCAACTTCTGCCCCCCCCATATAAGAA351390.18963482869562065No Hit
GCATAGCTCTTAAACGCGTAAGAATAACAGTGGTCCCCCCATATAAGAA349630.18868500855701598No Hit
GCATAGCTCTTAAACAGCGCTTCTTTATTGTTCCCCCCCCATATAAGAA349350.1885339008076925No Hit
GCATAGCTCTTAAACCGGTGTAGATCAGGCGGCCCCCCATATAAGAAAC279570.15087569099415085No Hit
GCATAGCTCTTAAACCGGTGTAGATCAGGCGGCCCCCCATATAAGAAAG266580.14386537076660846No Hit
GCATAGCTCTTAAACCGGTGTAGATCAGGCGGCACCCCCATATAAGAAA263750.14233810315737483No Hit
GCATAGCTCTTAAACGGACTATCATGATCTCGGTTCCCCCATATAAGAA235170.12691431931571504No Hit
GCATAGCTCTTAAACCGGTGTAGATCAGGCGGCCCCCCATATAAGAAAA215350.11621804934574662No Hit
GCATAGCTCTTAAACAACTTCACTCTTTGGACAGCCCCATATAAGAAAC214390.11569996563378043No Hit
GCATAGCTCTTAAACGGATATAGTCAACAGGATTCCCCCCATATAAGAA210870.11380032535657113No Hit
GCATAGCTCTTAAACAACTTCACTCTTTGGACAGCCCCCCATATAAGAA188990.10199233408800862No Hit
GCATAGCTCTTAAACGCGTAAGAATAACAGTGGTCCCCATATAAGAAAG186750.10078347209342085No Hit
GCATAGCTCTTAAACAACTTCACTCTTTGGACAGCCCCATATAAGAAAG186640.10072410833475806No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATTAG900.043.0149351
GCCAAGC255.081012E-543.014931
GCATCTC208.786462E-443.014931
CTAAGGT456.020855E-1043.0034415
CCCGTGC900.043.0034415
CCACTGG4650.043.0034415
CAATAGT1150.043.0034415
CAAACGT600.043.00343715
CGAACGT1850.043.00343715
CGGCCTA1100.043.00343715
CACATGT208.7980286E-443.00343715
CAATTGG503.6379788E-1143.00343715
CGATTGT950.043.00343715
CTGACGT255.0890303E-543.00343715
CTTGCAC503.6379788E-1143.00343715
CCCTTGC302.9616513E-643.00343715
CCCTTGA401.0191798E-843.00343715
CACGTGT850.043.00343715
CCGTTTC1450.043.00343715
ACCTTTC255.0890303E-543.00343715
Produced by FastQC (version 0.11.5)