FastQCFastQC Report
Wed 28 Aug 2019
H5WYNBGXC_n02_GDO_BRD4_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5WYNBGXC_n02_GDO_BRD4_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33958175
Sequences flagged as poor quality0
Sequence length49
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATAGCTCTTAAACCGGTGTAGATCAGGCGGCCCCCCCATATAAGAAA33455889.852084218306786No Hit
GCATAGCTCTTAAACTTGCGACGCTTAGCCTCCGTCCCCATATAAGAAA26299617.744706539736014No Hit
GCATAGCTCTTAAACCGTAGCTGAGAGTTGGTGCTCCCCATATAAGAAA25053137.377643233183173No Hit
GCATAGCTCTTAAACAACTTCACTCTTTGGACAGCCCCCATATAAGAAA16057574.728631618159692No Hit
GCATAGCTCTTAAACCATTGAATTCGCCTACACGGCCCCATATAAGAAA14078684.145888287577291No Hit
GCATAGCTCTTAAACGCGTAAGAATAACAGTGGTCCCCCATATAAGAAA12790333.7664951075845505No Hit
GCATAGCTCTTAAACGGACTATCATGATCTCGGTTCCCCATATAAGAAA12098873.5628740354863004No Hit
GCATAGCTCTTAAACACACGACTACTGTGACATCACCCCATATAAGAAA11923503.5112310953106283No Hit
GCATAGCTCTTAAACAGCGCTTCTTTATTGTTCCCCCCCATATAAGAAA10349913.047840468458626No Hit
GCATAGCTCTTAAACCACTGTTGTGGTGGATGCAGCCCCATATAAGAAA8515212.507558194749865No Hit
GCATAGCTCTTAAACTTGAACGGGCCGCGGAAGCGCCCCATATAAGAAA8374472.466113093533442No Hit
GCATAGCTCTTAAACCGCCGTTAAGCGGAAACGATCCCCATATAAGAAA7649842.2527241231308808No Hit
GCATAGCTCTTAAACAGATCTATATCTTGCCTCCACCCCATATAAGAAA7366692.1693421392639625No Hit
GCATAGCTCTTAAACAATTGTAGACGTTCTCCACACCCCATATAAGAAA7289922.146734917291639No Hit
GCATAGCTCTTAAACCTGTTGATTTAGACGGTATGCCCCATATAAGAAA6253411.8415035554767003No Hit
GCATAGCTCTTAAACGGATATAGTCAACAGGATTCCCCCATATAAGAAA5497411.6188767505909845No Hit
GCATAGCTCTTAAACCCGGCAGAAATGTCGAGTGGCCCCATATAAGAAA5404221.5914341686501114No Hit
GCATAGCTCTTAAACCCCTGTTGTTGACTTCAACGCCCCATATAAGAAA5159141.5192630346006522No Hit
GCATAGCTCTTAAACTAACGTACAACTTGATCTCGCCCCATATAAGAAA5021581.4787543794682725No Hit
GCATAGCTCTTAAACCACTGAATTCTCAGTACTGTCCCCATATAAGAAA4930821.4520273836859607No Hit
GCATAGCTCTTAAACCTCGGGAGAAAGAGGAGCGTCCCCATATAAGAAA4861141.4315080242091927No Hit
GCATAGCTCTTAAACACGTATGAGTCGGAGGAAGACCCCATATAAGAAA3811021.1222687909465099No Hit
GCATAGCTCTTAAACCGGTGTAGATCAGGCGGCCCCCCCCATATAAGAA3215400.946870672525835No Hit
GCATAGCTCTTAAACCCTTTCTGATATCCAGTTTACCCCATATAAGAAA3159430.9303886324868754No Hit
GCATAGCTCTTAAACCAGGACAGCAACTTCTGCCCCCCCATATAAGAAA3069150.9038029870568722No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2548700.7505409227674927No Hit
GCATAGCTCTTAAACCCCAGTTCATGGCCCACAAGCCCCATATAAGAAA1841320.542231730651014No Hit
GCATAGCTCTTAAACCGGTGTAGATCACGCTGCACCCCCAAAAAATAAA1551980.45702691619911845No Hit
GCATAGCTCTTAAACCCGCGCCGCTCGGGAGAAAGCCCCATATAAGAAA1421220.4185207243911076No Hit
GCATAGCTCTTAAACACGTATATCCCGAATTAATGCCCCATATAAGAAA1063010.31303507918196427No Hit
GCATAGCTCTTAAACCGTAGCTGAGAGTTGGTGCTCCCCCATATAAGAA756980.2229153951883457No Hit
GCATAGCTCTTAAACAGCGCTTCTTTATTGTTCCCCCCCCATATAAGAA716120.21088294644809386No Hit
GCATAGCTCTTAAACGCGTAAGAATAACAGTGGTCCCCCCATATAAGAA652670.1921981967523284No Hit
GCATAGCTCTTAAACCAGGACAGCAACTTCTGCCCCCCCCATATAAGAA561640.16539169139684332No Hit
GCATAGCTCTTAAACCGGTGTAGATCAGGCGGCCCCCCATATAAGAAAC477460.14060237336075923No Hit
GCATAGCTCTTAAACCGGTGTAGATCAGGCGGCCCCCCATATAAGAAAG473760.13951279772838204No Hit
GCATAGCTCTTAAACCCGTAAGAATAACAGTGGTCCCCCATATAAGAAA454060.13371154368572516No Hit
GCATAGCTCTTAAACCGGTGTAGATCAGGCGGCACCCCCATATAAGAAA442750.1303809760094587No Hit
GCATAGCTCTTAAACCGTAGCTGAGAGTTGGTGCTCCCATATAAGAAAC423280.12464745234394958No Hit
GCATAGCTCTTAAACCAGGACAGCAACTTCTGTCCCCCCATATAAGAAA413740.1218381140918203No Hit
GCATAGCTCTTAAACAACTTCACTCTTTGGACAGCCCCATATAAGAAAC402950.11866067596388793No Hit
GCATAGCTCTTAAACCGTAGCTGAGAGTTGGTGCTCCCATATAAGAAAG400420.11791564181526246No Hit
GCATAGCTCTTAAACTTGCGACGCTTAGCCTCCGTCCCCCATATAAGAA395940.11659637186038413No Hit
GCATAGCTCTTAAACAACTTCACTCTTTGGACAGCCCCATATAAGAAAG390290.1149325604217541No Hit
GCATAGCTCTTAAACGGACTATCATGATCTCGGTTCCCCCATATAAGAA377410.11113965930147895No Hit
GCATAGCTCTTAAACCGGTGTAGATCAGGCGGCCCCCCATATAAGAAAA377020.1110248121402284No Hit
GCATAGCTCTTAAACCATTGAATTCGCCTACACGGCCCCCATATAAGAA366910.1080476203447329No Hit
GCATAGCTCTTAAACAATTGTAGACGTTCTCCACACCCCCATATAAGAA348840.10272636854012325No Hit
GCATAGCTCTTAAACAACTTCACTCTTTGGACAGCCCCATATAAGAAAA343380.1011185082826153No Hit
GCATAGCTCTTAAACGCGTAAGAATAACAGTGGTCCCCATATAAGAAAG339680.10002893265023813No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAATAG2300.043.0153581
TGGACTA255.0890263E-543.00357415
CCAATGT1650.043.00357415
ACCTGTC351.7332059E-743.00357414
ACCAGTC255.0890263E-543.00357414
CCTATGT302.9616604E-643.00357415
CCTATGG208.797996E-443.00357415
CATCGGT302.9618404E-643.0031932
CCTTTCG351.7334787E-743.0026916
TTGCGAA6550.043.0026916
GGGTCTA503.6379788E-1143.00268616
TAACGTC208.7988895E-443.00268616
CAATGTA401.0193617E-843.00268616
TACGGGT255.0898227E-543.0024313
TACCTGT302.9622006E-643.0024313
TTGTATT351.7336242E-743.00224718
GTGCATT503.6379788E-1143.00224318
CTGTCAT1150.043.00224319
GCGACTC2950.043.00224318
GGGTCAT302.9622897E-643.00224319
Produced by FastQC (version 0.11.5)