FastQCFastQC Report
Wed 28 Aug 2019
H5WYNBGXC_n02_BRD4_bulk.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5WYNBGXC_n02_BRD4_bulk.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31460272
Sequences flagged as poor quality0
Sequence length49
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGTACTCTGCGTTGATACCACTGCTTCCGCGGACAGGCGTGTAGATC471300.14980798640266046No Hit
CACGTACTCTGCGTTGATACCACTGCTTCCGCGGACAGGCGTGTAGATC396750.12611143349300985No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGATC165750.030.4305143
TGTAGAT254450.020.81916642
GTATCAA222700.020.1174531
GGTATCA95100.017.3920081
ACTTACG120800.017.2825217
CTTACGT122450.016.9093318
GTGTAGA332000.015.88485141
AGGTACG53050.015.80643717
GGCACTT133100.015.65367714
GCACTTA134750.015.65350415
CGTGTAG338150.015.37985640
TTACGTA170950.015.05525619
TCAACGC286050.014.8514213
GCGTGTA355200.014.53905539
CAACGCA293450.014.4476494
GCACGTA69950.014.26242319
CGAGCGT54300.014.2541126
GGTACGT59200.014.23715618
ATCAACG311600.013.8205892
TATCAAC315800.013.6282121
Produced by FastQC (version 0.11.5)