FastQCFastQC Report
Wed 28 Aug 2019
H5WYNBGXC_n02_BLG_BRD4_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5WYNBGXC_n02_BLG_BRD4_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29985224
Sequences flagged as poor quality0
Sequence length49
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5461091.821260364771662No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTCCG19263050.042.52530312
TGTCCGA19312000.042.50031713
GTCCGAC19330600.042.48006414
TCCGACA19405200.042.46678515
GTCTTTG6118250.042.45587511
CCGACAA19449000.042.42702516
AGTCTTT1946600.042.4228610
CGACAAT19455700.042.3757117
GGTCTTT2148800.042.36025210
GACAATA19524200.042.355318
TTTGTCA6128200.042.33054414
TCTTTGT6133400.042.32330312
AACGCTT986950.042.31793613
GTTGTCC19360200.042.30395511
ACAATAC19549750.042.3021319
TGTTGTC5532500.042.26285610
TCAACGC991300.042.2421511
AGTTGTC4790250.042.23994410
CAATACT4900700.042.23044220
TCCTACC1380300.042.22625713