FastQCFastQC Report
Wed 28 Aug 2019
H5WYNBGXC_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5WYNBGXC_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33324537
Sequences flagged as poor quality0
Sequence length26
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAA389530.11688984606147716No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTGAG486600.019.611832
CGATTGT499800.019.11418710
GTTTTTT1302700.018.9937820
TCGATTG503850.018.9685389
ATTTTTT863600.018.87891820
GAGGAAC107850.018.869771
GAACTAC132500.018.8060425
GATTTTT275900.018.80267519
TGAGCTA123600.018.7365326
TCCTTAC169800.018.7334294
CAGAGCG129200.018.7233542
GAACTGG92300.018.720236
ATTACCT283900.018.7196129
ACACTGA529200.018.6841661
GTCGATT512300.018.6773288
GGAACAG114600.018.6593783
ACTGAGT513300.018.5868433
ACGGTAC114300.018.5634692
AGTCGAT510500.018.52977
AGTGCTC116500.018.518183
Produced by FastQC (version 0.11.5)