FastQCFastQC Report
Wed 28 Aug 2019
H5WYNBGXC_n01_cDNA_BRD4_2d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5WYNBGXC_n01_cDNA_BRD4_2d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37243303
Sequences flagged as poor quality0
Sequence length26
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTAAC108600.017.6936051
TGCCAAA152150.017.6650351
CTCGAAA103250.017.6513161
GATGAAA85750.017.6258131
TGACTAG112350.017.4413531
ATAACGC139000.017.2493231
ACTGAAC84150.017.200191
GAATGAA77500.017.1272641
CTGATAG123550.017.1232591
AACGAAA70100.017.08924510
CAATGGA97150.017.086658
CGGACTG115800.016.9821911
GGGACCT96850.016.9793471
GGGAATG95900.016.928511
TACGGAT113150.016.9025461
ATGCGAT92100.016.8884411
CTTAGGA119550.016.8511161
GAACCTA134400.016.8126351
AGCTTGA112750.016.740721
ACGAGGA93750.016.729911
Produced by FastQC (version 0.11.5)