FastQCFastQC Report
Wed 28 Aug 2019
H5WYNBGXC_n01_cDNA_BRD4_2c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5WYNBGXC_n01_cDNA_BRD4_2c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36924392
Sequences flagged as poor quality0
Sequence length26
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTAAC109550.017.9789331
CTCGAAA99500.017.8948291
TGCCAAA150150.017.5543981
GATGAAA87350.017.5324611
TGACTAG113000.017.4565771
ATAACGC135150.017.3415071
AGCTTGA109100.017.2829251
ACTGAAC85050.017.2185821
ATGCGAT88900.017.181771
CGGACTG113650.017.0750871
CAATGGA97250.017.0172588
GAATGAA76050.017.0070881
GGGAATG100050.016.9466251
CTGATAG121400.016.9326041
ATCGAGT100150.016.8497981
TACGGAT115700.016.781191
TTGAACG94150.016.7761551
AACGAAA68100.016.76848210
CACCGAA68850.016.7313028
GAACCTA134200.016.718891
Produced by FastQC (version 0.11.5)