FastQCFastQC Report
Wed 28 Aug 2019
H5WYNBGXC_n01_cDNA_BRD4_2b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5WYNBGXC_n01_cDNA_BRD4_2b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35191359
Sequences flagged as poor quality0
Sequence length26
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTAAC108650.017.7226281
CTCGAAA92800.017.6883641
TGCCAAA136050.017.660441
GATGAAA81350.017.6203981
AACGAAA69700.017.34495210
CAATGGA90350.017.343078
CTGATAG111400.017.2941071
ACTGAAC80250.017.2511541
ATAACGC134100.017.1936951
TGACTAG107100.017.1011731
GGAAAGC93600.017.0883331
CGGACTG107600.017.0774881
ATGCGAT85100.017.0437661
AGCTTGA106550.016.9923021
GAACCTA122950.016.9468041
TACGGAT105900.016.9454671
GAATGAA75500.016.9056071
GGGAATG93500.016.8819451
ACGAGGA85600.016.7922951
ACGGAGA117000.016.739941
Produced by FastQC (version 0.11.5)