FastQCFastQC Report
Wed 28 Aug 2019
H5WYNBGXC_n01_cDNA_BRD4_2a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5WYNBGXC_n01_cDNA_BRD4_2a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38468738
Sequences flagged as poor quality0
Sequence length26
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTAAC114800.017.697271
TGCCAAA154150.017.6702351
CTCGAAA105800.017.5386641
GATGAAA87750.017.5098461
GAATGAA80050.017.3446831
TGACTAG118950.017.3321231
ACTGAAC85950.017.3179021
CGGACTG124900.017.3073481
ATAACGC139600.017.3049341
GAACCTA133950.017.183521
AACGAAA73800.017.11277210
AGCTTGA114650.017.100951
CTGATAG126950.017.0357481
ATGCGAT90200.017.0120641
GGGAATG102450.016.9502451
CACCGAA70350.016.9429028
GGGACCT99750.016.937721
CAATGGA102800.016.925128
CCGGAAA58250.016.73717110
TACGGAT122600.016.7182081
Produced by FastQC (version 0.11.5)