FastQCFastQC Report
Wed 28 Aug 2019
H5WYNBGXC_n01_cDNA_BRD4_1d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5WYNBGXC_n01_cDNA_BRD4_1d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18445295
Sequences flagged as poor quality0
Sequence length26
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTAAC49850.018.0993141
CAATGGA76050.017.9347218
ATGCGAT59050.017.7356681
GGAAAGC44000.017.45941
GATGAAA37600.017.3984551
CACCGAA36250.017.3509038
GATGCTA62600.017.2891521
TGACTAG53300.017.2280541
AGACTAG51750.017.2165768
GGGACCT51050.017.1644211
CGGACTG65400.017.1607341
CGGAGCT52600.017.1150251
CTGCGGA52050.017.0652641
ACTGAAC47300.017.0449091
ATTGGAC78150.017.0104871
ATGGGAG56150.016.870231
AAGAAAA21500.016.8399282
TACGGAT45600.016.7809811
CTCGAAA38500.016.7319241
TGAGCAT55300.016.7315961
Produced by FastQC (version 0.11.5)