FastQCFastQC Report
Wed 28 Aug 2019
H5WYNBGXC_n01_cDNA_BRD4_1c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5WYNBGXC_n01_cDNA_BRD4_1c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18001014
Sequences flagged as poor quality0
Sequence length26
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCGAT59300.017.8812581
CAATGGA69550.017.7848648
CGGACTG60400.017.6549781
ATTGGAC74400.017.6134851
GATGCTA59450.017.5837441
GACTAAC47300.017.468911
CTCGAAA39250.017.4581341
AGACTAG49850.017.3511778
GGGAATG40650.017.2506071
GATGAAA34600.017.1445261
AAGAAAA19700.017.1093432
GGAAAGC42200.017.0910871
CACCGAA35550.017.0736798
GGTATTG58800.016.978531
CGGAGCT56050.016.9548871
CTGCGGA47900.016.9368361
GAAATGA50850.016.8985371
ACTGAAC42550.016.8329541
ATGGGAG52300.016.7934421
GGAACTT58150.016.7382491
Produced by FastQC (version 0.11.5)