FastQCFastQC Report
Wed 28 Aug 2019
H5WYNBGXC_n01_cDNA_BRD4_1b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5WYNBGXC_n01_cDNA_BRD4_1b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31048776
Sequences flagged as poor quality0
Sequence length26
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATGGA123850.017.9323888
ATGCGAT97350.017.8682271
GACTAAC83150.017.7197781
ACTGAAC76600.017.485161
GATGCTA103250.017.3509541
AAGAAAA34150.017.3376982
GATGAAA59900.017.2333891
TGACTAG86550.017.1970391
AGACTAG86600.017.1818778
CGGACTG111000.017.1487961
ATTGGAC137100.017.1016461
CGGAGCT90900.017.0673351
CTCGAAA63650.017.0352571
GGAAAGC72450.016.9956381
GGGAATG71750.016.9662721
CTGCGGA85200.016.9294761
CAATGAA44250.016.8581988
GAAATGA81900.016.8543871
CACCGAA61800.016.8441038
TGAGCAT88700.016.8253081
Produced by FastQC (version 0.11.5)