FastQCFastQC Report
Wed 28 Aug 2019
H5WYNBGXC_n01_cDNA_BRD4_1a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5WYNBGXC_n01_cDNA_BRD4_1a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18158415
Sequences flagged as poor quality0
Sequence length26
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATGGA72800.017.6781778
GATGCTA60150.017.6608661
GACTAAC46000.017.6354311
ACTGAAC45950.017.5675471
CGGACTG63550.017.5659521
ATGCGAT58100.017.5264821
GGGAATG43000.017.1443921
ATTGGAC80100.017.1334441
ATGGGAG55000.017.0411931
TGACTAG52150.017.032631
AGACTAG53650.017.0173268
CTCGAAA38250.016.972131
CACCGAA33050.016.9436478
GGAACTT59900.016.8996141
GATGAAA33850.016.8142191
TTGGAAC37300.016.7875981
ATCCGAA61000.016.7588481
GGAAAGC44150.016.7431351
GGGACCT48250.016.730131
GAAATGA49100.016.66461
Produced by FastQC (version 0.11.5)