FastQCFastQC Report
Wed 28 Aug 2019
H5WYNBGXC_n01_HTO_BRD4_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5WYNBGXC_n01_HTO_BRD4_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8893576
Sequences flagged as poor quality0
Sequence length26
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAATA186850.019.3736327
TCAATAA187750.019.3446798
AATAAGG188150.019.30832310
TAATCAA186750.019.294175
AATCAAT187450.019.2703536
GTACTCC207600.019.2100851
TCGGTAA197100.019.1931081
CCTCTGG197850.019.1065856
GAATGTA73600.019.0654162
TACTCCT201600.019.0604552
TGGTATG205250.019.04921210
ACTCCTC202400.019.0330353
AATGTAG73800.019.0258033
ATAAGGC69950.018.96986211
TCCTCTG199700.018.8943185
GTAATCA192200.018.8620684
CGGTAAT193550.018.8455582
TTAAGAT100050.018.7700029
GGGCATG77550.018.6583219
GGTAATC195750.018.632293
Produced by FastQC (version 0.11.5)