FastQCFastQC Report
Wed 28 Aug 2019
H5WYNBGXC_n01_HTO_BRD4_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5WYNBGXC_n01_HTO_BRD4_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11897623
Sequences flagged as poor quality0
Sequence length26
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACTGGT1607950.019.8658031
ACTGGTG1584050.019.7128222
TGGTGTT1595500.019.6198844
TGTTGCC1589600.019.6150677
GTGTTGC1591850.019.5864736
TTGCCTC1604500.019.5669089
AGCAACA351950.019.552653
GTTGCCT1607900.019.5261548
CTGGTGT1610400.019.5246753
TGCCTCT1608250.019.51000810
GGTGTTG1601550.019.4928745
CGTGAGA355600.019.47911510
CACGTGA356600.019.4329838
ACGTGAG357550.019.3869449
CGCGCAG229550.019.3219684
TAGTGGT389700.019.3069931
GCCACCT383200.019.268769
GGAACTT149950.019.2446981
GAAAAGA55450.019.2441144
AAAGAAA52700.019.2230786
Produced by FastQC (version 0.11.5)