FastQCFastQC Report
Wed 28 Aug 2019
H5WYNBGXC_n01_GDO_BRD4_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5WYNBGXC_n01_GDO_BRD4_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18529824
Sequences flagged as poor quality0
Sequence length26
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGAAAA57300.019.2704732
GAAAAGA57550.019.0638224
AGAAAAG58750.018.7935433
AAAAGAC57450.018.6608875
TGCCAAA75700.018.5396651
GCGAAGA74350.018.4553284
AACGAAA64400.018.3529210
TGACTAG77450.018.2886621
GATGAAA38950.018.2857441
GAAGACT66650.018.2311556
CTCGAAA48250.018.2234941
CTGATAG79700.018.1865431
GCAGGAA42150.018.1248847
GGGACCT78700.018.0108911
ACTGAAC53550.018.0076751
GGACGAA32500.017.937429
TTAAAGA46950.017.9116349
GCACATA121000.017.8239291
GAACGGA105450.017.7961621
GGACCAC70800.017.7814549
Produced by FastQC (version 0.11.5)