FastQCFastQC Report
Wed 28 Aug 2019
H5WYNBGXC_n01_GDO_BRD4_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5WYNBGXC_n01_GDO_BRD4_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33958175
Sequences flagged as poor quality0
Sequence length26
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGCAC423800.019.1018241
AGACTAG243650.018.9608828
CTCGAAA149500.018.9151971
GGAATAA195800.018.6724341
CAATAAC157100.018.5925248
GACTAAC121850.018.5035321
ATAACGA158000.018.45481110
TACACGA215650.018.419451
TGGACGC217550.018.4103161
CACGAGT209850.018.405423
AGATAAG129600.018.34788310
ATTGGAC215250.018.342151
AATAACG159250.018.3225389
AGCTACA95600.018.317693
ATCGAGA134800.018.3079157
GAAGAAA44100.018.3010036
CACAGAA118300.018.2512975
GGACGCA158100.018.2255542
CTTAACT198500.018.1866461
GAGAACG162500.018.14682210
Produced by FastQC (version 0.11.5)