FastQCFastQC Report
Wed 28 Aug 2019
H5WYNBGXC_n01_BRD4_bulk.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5WYNBGXC_n01_BRD4_bulk.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31460272
Sequences flagged as poor quality0
Sequence length26
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTGTCTCTTATACACATCTCCGAGC539040.1713399044992364No Hit
GCTGTCTCTTATACACATCTCCGAGC462550.1470267008498846No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCAGC1354900.019.9663331
AACACCA1440800.019.9661582
GAACACC1442750.019.9555421
TCAGAAC1279500.019.9513572
GACTCTA1501550.019.9508361
AAGTGCC1512200.019.950561
AACTACC1277100.019.9410386
TCCTTAC1627700.019.9359514
AGAACTA1279200.019.9358544
TCAGAGC1345600.019.9333531
CTCTATG1503950.019.9331843
TTCAGCA1355050.019.929142
TCTCCTA1216350.019.9283433
GAATCCT1632250.019.9282441
CACCATA1440050.019.9280014
ATCCTTA1630150.019.9250413
CTCCTAC1216150.019.9233674
AACGTGC1256300.019.9226551
TCAGCAA1355900.019.9197333
GAACTAC1280050.019.9125465
Produced by FastQC (version 0.11.5)