FastQCFastQC Report
Wed 28 Aug 2019
H5WYNBGXC_n01_BLG_BRD4_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5WYNBGXC_n01_BLG_BRD4_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29985224
Sequences flagged as poor quality0
Sequence length26
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTGA9030500.019.954841
CAGTAAC3136000.019.8576681
CGACTTC1981050.019.816511
CACTGAG9095450.019.8134382
AGTAACC3112700.019.8095822
GACTTCT1973200.019.8002662
ACGCCAG2286200.019.7852731
CCAGCGA1304400.019.7683941
CGCCAGC2304100.019.7477762
GTAACCA3117950.019.7442463
CCAGCAA2303550.019.7322374
AAGCCTA1953650.019.73148310
GCCAGCA2310350.019.7187123
CAGCAAG2307150.019.7127295
TAACCAT3123450.019.703754
GCAAGTC2308000.019.6775937
ACTGAGT9164550.019.6709423
AACCATT3133100.019.6667885
AGCAAGT2311800.019.6659476
CAAGCCT1962700.019.6390729
Produced by FastQC (version 0.11.5)