FastQCFastQC Report
Wed 28 Aug 2019
H5WYNBGXC_n01_BLG_BRD4_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5WYNBGXC_n01_BLG_BRD4_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29471589
Sequences flagged as poor quality0
Sequence length26
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGACTT7763500.019.8361
TGACTTC7850350.019.7292882
GACTTCA7841450.019.7127553
CATTACC7869700.019.6586468
ATTACCT7854150.019.6390239
ACTTCAT7875900.019.6287884
TTACCTT7867500.019.59171710
CTAGTGA1109900.019.5712931
TCATTAC7909550.019.548987
TTCATTA7912600.019.5359926
ACCGAGG520250.019.5060254
TAGTGAA1097550.019.5037062
TACCTTC2343950.019.4899911
GAAGTGA1101250.019.4667246
TACCTTT2482950.019.45203411
CTTCATT7955600.019.4250955
GAGGCCC524000.019.4210837
AAGTGAA1109100.019.4186767
CAATGGA778550.019.3879368
TACCTTG1506100.019.38745311
Produced by FastQC (version 0.11.5)