FastQCFastQC Report
Tue 21 May 2019
H5WNTBCX2_l02.1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5WNTBCX2_l02.1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences223221549
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGA100296264.493126243828726Illumina Paired End PCR Primer 2 (100% over 32bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGC5098050.22838520845494176Illumina Paired End PCR Primer 2 (97% over 36bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGG3437090.1539766216746395Illumina Paired End PCR Primer 2 (96% over 33bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGAG13129300.037.46426815
CCGAGAT13174700.036.94635817
CGAGATC13295200.036.60231418
GCCGAGA13724200.035.8806416
GCGGTTC14563550.033.05529431
AGCGGTT14683650.032.83205830
GAGATCG14991400.032.4594519
GAGCGGT14927250.032.29065329
AGAGCGG15261600.031.6215428
AAGAGCG15460950.031.20621327
GAAGAGC16260650.029.71050526
TCGGAAG16365100.029.55303223
CGGAAGA16502700.029.3182724
ATCGGAA16687600.029.02944622
AGATCGG17113550.028.38202720
GATCGGA17139750.028.29790521
GGAAGAG17206000.028.13898325
ATGCCGA23567650.020.84467514
AATGCCG23737300.020.69642313
CTCGTAT249800.020.47154628
Produced by FastQC (version 0.11.5)