FastQCFastQC Report
Tue 21 May 2019
H5WNTBCX2_l01.1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5WNTBCX2_l01.1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences118657554
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGA9719030.8190822810994403Illumina Paired End PCR Primer 2 (100% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGTTC1485700.040.0840331
AGCGGTT1491400.040.0054130
GAGCGGT1494900.039.7920529
TGCCGAG1617200.038.88591415
AGAGCGG1554900.038.3013128
AAGAGCG1552150.038.24194327
CGGAAGA1633100.036.68224324
CGAGATC1698350.036.54236218
TCGGAAG1644400.036.354423
ATCGGAA1666550.036.12889522
GATCGGA1700800.035.2220621
CCGAGAT1772300.035.20316717
GCCGAGA1843050.034.33666616
AGATCGG1796650.033.39042320
GAAGAGC1861500.032.16117526
GAGATCG1908450.032.15027219
GGAAGAG2153500.027.60349325
CGGTTCA2925650.022.155861
ATGCCGA2918150.021.78542114
AATGCCG2932350.021.56700913
Produced by FastQC (version 0.11.5)