FastQCFastQC Report
Fri 23 Dec 2016
H5WJNBCXY_l02n02_handr_mekmut_18hpf_10_4.3520000007481e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5WJNBCXY_l02n02_handr_mekmut_18hpf_10_4.3520000007481e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3799555
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCGT2800.016.8659296
TATACAC83550.014.31120645
TTATACA125950.012.76582645
CTTATAC157500.012.03465545
TGGACCG3753.6379788E-1111.4171625
GTCTTAT7250.011.1973531
TATACCG6650.011.1822215
GTCCTAG2901.6361082E-710.8863161
TACTCCG3359.591531E-910.7624455
ACCGTGC6350.010.62420758
ATACACA58850.010.543694545
TACACTG12400.010.5400365
TACACCG7700.010.53531555
TCTAGGC5350.010.530683
TCTTATA209550.010.30978145
TAGACAG7000.010.3011985
GTCCTAA3951.0786607E-910.2760521
CCTACAC8850.010.1856063
TCTGACG12700.010.16388845
CCGTGCG4253.765308E-1010.0534179
Produced by FastQC (version 0.11.2)