FastQCFastQC Report
Fri 23 Dec 2016
H5WJNBCXY_l02n02_handr_dnfgfr_18hpf_10_6.352000000747f3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5WJNBCXY_l02n02_handr_dnfgfr_18hpf_10_6.352000000747f3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10189587
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACAC239200.014.02992745
TTATACA339550.012.65787645
ATACACA163950.012.00263845
TAGACCG18950.011.6522675
TATACCG20050.011.5748785
CTTATAC429900.011.29342645
CCGTCCG8600.010.9825699
ACCGTGC20350.010.6086458
TACACTG36450.010.4467875
TCTTATA553750.010.4401245
TACACAT106100.010.41532445
GGACCAT11400.010.2577386
TATACTG26850.010.2378595
GTATAGG29800.010.2141841
ACCGTCC14500.010.0808818
TACACCG21200.09.9904615
AGACCGT22300.09.9835156
GTGCTAG16200.09.8816521
GTATAGA25050.09.8108121
CCGTCCC9000.09.7448519
Produced by FastQC (version 0.11.2)