FastQCFastQC Report
Fri 23 Dec 2016
H5WJNBCXY_l02n02_gfp2_18hpf_12_08.35200000074838.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5WJNBCXY_l02n02_gfp2_18hpf_12_08.35200000074838.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48200324
Sequences flagged as poor quality0
Sequence length51
%GC34

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGGT67400.021.25341241
CTGCCGA72100.019.83644324
CTCGGTG71400.019.71629342
TCGGTGG74350.019.26671443
CCGACGA74200.019.063627
GACGAGT75400.018.79006629
GCTGCCG80400.018.0123723
AGTCTGA107700.017.4556933
AACCGCG80950.017.251337
CGCTGCC85350.017.09945322
ATCTCGG84600.016.79963940
GCCGACG85350.016.704926
TGCCGAC87200.016.65936525
ACGAGTG87800.016.34124830
CGAGTGT92000.015.83967331
ACGCTGC93800.015.58300121
TAGATAG86550.015.3343065
GCGGTAG84500.015.16906311
CGTCCAG78400.014.85779210
GTGTAGA98750.014.71124834
Produced by FastQC (version 0.11.2)