FastQCFastQC Report
Fri 23 Dec 2016
H5WJNBCXY_l02n01_handr_mekmut_18hpf_10_4.35100000074811.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5WJNBCXY_l02n01_handr_mekmut_18hpf_10_4.35100000074811.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3799555
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACAC86250.014.03503345
CTAGGGC3201.8189894E-1213.3576783
TTATACA125800.012.89569945
TCTCCGA10200.012.1325745
CTTATAC159100.011.93610345
CTCCGAG5600.011.65199445
CCGTGCG4300.011.5112289
ACCGTCC5100.011.4701898
TAGGACT3551.7462298E-1011.4070034
CCTACAC10850.011.1967353
ATCTCCG13300.010.99643745
ACCGTGC5950.010.9660048
GTCTTAG4509.094947E-1210.5335991
ATACACA61100.010.49527745
GGACTGC4751.8189894E-1210.4205536
GTCCTAA3908.4946805E-1010.4178451
TCTTATA210200.010.39385845
TCCTACA8850.010.16820052
GTAAGAC6650.010.1490893
TAGACCG5850.09.9992585
Produced by FastQC (version 0.11.2)