FastQCFastQC Report
Fri 23 Dec 2016
H5WJNBCXY_l02n01_handr_mekmut1_18hpf_12_13.351000000747dc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5WJNBCXY_l02n01_handr_mekmut1_18hpf_12_13.351000000747dc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences102893641
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG423650.011.0511395
CGGGACT221700.010.55449110
AACCGCG156550.010.0172687
CTCCGAG122900.010.0147245
TCGGGAC234100.09.9762099
ATCTCCG264600.09.86441945
TATACCG243300.09.7278265
TACACTG401250.09.6383575
TATACAC1851350.09.4338445
CGTCCAG106600.09.3923110
TAGACCG173300.09.386035
GTATAAG481850.09.3452811
CTAATAC374400.09.3376623
GGACGAC145400.09.1606647
CTGGACG132700.09.1212335
TAGGTCG174250.08.8829625
CTATACT359350.08.8710544
CTTATAC2689950.08.86759845
ACCGCGG144850.08.85372458
GTCTTAG120600.08.8152551
Produced by FastQC (version 0.11.2)