FastQCFastQC Report
Fri 23 Dec 2016
H5WJNBCXY_l02n01_handr_dnfgfr_18hpf_12_9_2000_cells.35100000074855.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5WJNBCXY_l02n01_handr_dnfgfr_18hpf_12_9_2000_cells.35100000074855.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15886697
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACCG33250.011.8409575
ACGGTAC22150.011.7828948
ACCGTGC16350.011.2840198
TACACCG26700.010.8697325
TACACTG65200.010.662315
TATACAG78000.010.6431995
CTTATAC336500.010.53183945
AGACCGT37450.010.5131646
TAGACGG11400.010.4595125
TCTATAC49000.010.4219673
TATACTG69300.010.4210675
TATACCG33950.010.4039975
ACCGCTC17950.010.02751358
CTAGACG9900.09.9985574
GTATAGG55050.09.9224031
CCTACAC44700.09.6630353
CCTTTGG57350.09.6031841
AGTACCG15700.09.6009795
CCGTGCG12250.09.5506959
CGTATAC30650.09.4684553
Produced by FastQC (version 0.11.2)