FastQCFastQC Report
Fri 23 Dec 2016
H5WJNBCXY_l02n01_gfp_10hpf_june_2.3510000007487e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5WJNBCXY_l02n01_gfp_10hpf_june_2.3510000007487e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1093
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAATTGTTCCAAGTATCGGCAACAGCTTTATCAATACCATGAAAAATATC30.2744739249771272No Hit
AAATAAAGTAATACTATTAAAATTCTTAGAAAAATTATATTTTTTATAAAG20.18298261665141813No Hit
TATTGAGGCTTGTGGCATTTCTACTCTTTCTCAATCCCCAATGCTTGGCTT20.18298261665141813No Hit
GTATTATAGATATTAATCTACCAACAACTCTAAAAGCACCTAAAGCACTAA20.18298261665141813No Hit
AAACTATATAGCGGGCAGTGAGTGCTTCAACCATAAAAATTCAAAACAATA20.18298261665141813No Hit
AGGTGTCTGTAAAACAGGTGCCGAAGAAGCTGGAGTAACAGAAGTGAGAAC20.18298261665141813No Hit
GTTCTTACTGACGCAGAAGAAAACGTGCGTCAAAAATTACGTGCAGAAGGA20.18298261665141813No Hit
ATCGGCAACAGCTTTATCAATACCATGAAAAATATCAACCACACCAGAAGC20.18298261665141813No Hit
CAAAAAGAGCTTACTAAAATGCAACTGGACAATCAGAAAGAGATTGCCGAG20.18298261665141813No Hit
AAGCAGGAATTACTACTGCTTGTTTACGAATTAAATCGAAGTGGACTGCTG20.18298261665141813No Hit
CACGTTTTCTTCTGCGTCAGTAAGAACGTCAGTGTTTCCTGCGCGTACACG20.18298261665141813No Hit
AAACAAAACTAGGGACGGCCTCATCAGGGTTAGGAACATTAGAGCCTTGAA20.18298261665141813No Hit
TGGCTGGAGACAAATAATCTCTTTAATAACCTGATTCAGCGAAACCAATCC20.18298261665141813No Hit
CAATGACGGCAGCAATAAACTCAACAGGAGCAGGAAAGCGAGGGTATCCTA20.18298261665141813No Hit
GTACCAATTTATAAAAAAAAAAAATAGAATAGAAAAGAAATCAATTAATTA20.18298261665141813No Hit
TCCCAAGAAGCTGTTCAGAATCAGAATGAGCCGCAACTTCGGGATGAAAAT20.18298261665141813No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)