FastQCFastQC Report
Fri 23 Dec 2016
H5WJNBCXY_l01n02_mesp_dnfgfr_10hpf_31march_2016.352000000747e6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5WJNBCXY_l01n02_mesp_dnfgfr_10hpf_31march_2016.352000000747e6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1940
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGTCAACATTCAAACGGCCTGTCTCATCATGGAAGGCGCTGAATTTACGG40.2061855670103093No Hit
GATTGGTGTTTTCCATAATAGACGCAACGCGAGCAGTAGACTCCTTCTGTT30.15463917525773196No Hit
AACTGGCGCCGAGCGTATGCCGCATGACCTTTCCCATCTTGGCTTCCTTGC30.15463917525773196No Hit
GGCGTCGCGTCGTAACCCAGCTTGGTAAGTTGGATTAAGCACTCCGTGGAC30.15463917525773196No Hit
AGAAAACGTGCGTCAAAAATTACGTGCAGAAGGAGTGATGTAATGTCTAAA30.15463917525773196No Hit
CCCTTACTTGAGGATAAATTATGTCTAATATTCAAACTGGCGCCGAGCGTA30.15463917525773196No Hit
GACCACTCGCGATTCAATCATGACTTCGTGATAAAAGATTGAGTGTGAGGT30.15463917525773196No Hit
TGAGTCTCATTTTGCATCTCGGCAATCTCTTTCTGATTGTCCAGTTGCATT30.15463917525773196No Hit
AATGCGACAGGCTCATGCTGATGGTTGGTTTATCGTTTTTGACACTCTCAC30.15463917525773196No Hit
AAGCGCCGTGGATGCCTGACCGTACCGAGGCTAACCCTAATGAGCTTAATC30.15463917525773196No Hit
CTGATGGTTGGTTTATCGTTTTTGACACTCTCACGTTGGCTGACGACCGAT30.15463917525773196No Hit
AATTTTAATTGCAGGGGCTTCGGCCCCTTACTTGAGGATAAATTATGTCTA20.10309278350515465No Hit
AAACGATAAACCAACCATCAGCATGAGCCTGTCGCATTGCATTCATCAAAC20.10309278350515465No Hit
AATTGGGAGGGTGTCAATCCTGACGGTTATTTCCTAGACAAATTAGAGCCA20.10309278350515465No Hit
ACCAACCATCAGCATGAGCCTGTCGCATTGCATTCATCAAACGCTGAATAG20.10309278350515465No Hit
TGCCGAGGGTCGCAAGGCTAATGATTCACACGCCGACTGCTATCAGTATTT20.10309278350515465No Hit
AAGATTGCTGAGGGTCAGTGGTATCGTTATGCGCCTTCGTATGTTTCTCCT20.10309278350515465No Hit
CGCGATTCAATCATGACTTCGTGATAAAAGATTGAGTGTGAGGTTATAACG20.10309278350515465No Hit
CGTCACGTTTATGGTGAACAGTGGATTAAGTTCATGAAGGATGGTGTTAAT20.10309278350515465No Hit
AATGTGACCGTTTATCGCAATCTGCCGACCACTCGCGATTCAATCATGACT20.10309278350515465No Hit
TGCTCGCGTTGCGTCTATTATGGAAAACACCAATCTTTCCAAGCAACAGCA20.10309278350515465No Hit
TAATGATTCACACGCCGACTGCTATCAGTATTTTTGTGTGCCTGAGTATGG20.10309278350515465No Hit
CCCTCTTAAGGATATTCGCGATGAGTATAATTACCCCAAAAAGAAAGGTAT20.10309278350515465No Hit
CCAAAACGGCAGAAGCCTGAATGAGCTTAATAGAGGCCAAAGCGGTCTGGA20.10309278350515465No Hit
ACTTCTGTTACTCCAGCTTCTTCGGCACCTGTTTTACAGACACCTAAAGCT20.10309278350515465No Hit
TAAAATGCAACTGGACAATCAGAAAGAGATTGCCGAGATGCAAAATGAGAC20.10309278350515465No Hit
CAATAATGTTTATGTTGGTTTCATGGTTTGGTCTAACTTTACCGCTACTAA20.10309278350515465No Hit
CCATAATAGACGCAACGCGAGCAGTAGACTCCTTCTGTTGATAAGCAAGCA20.10309278350515465No Hit
GTTCTGGCGCTCGCCCTGGTCGTCCGCAGCCGTTGCGAGGTACTAAAGGCA20.10309278350515465No Hit
ATATCTGGTTGAACGGCGTCGCGTCGTAACCCAGCTTGGTAAGTTGGATTA20.10309278350515465No Hit
AGCTAATGGCCGTCTTCATTTCCATGCGGTGCACTTTATGCGGACACTTCC20.10309278350515465No Hit
TATACTCATCGCGAATATCCTTAAGAGGGCGTTCAGCAGCCAGCTTGCGGC20.10309278350515465No Hit
TAAATCTGCCATTCAAGGCTCTAATGTTCCTAACCCTGATGAGGCCGTCCC20.10309278350515465No Hit
CGTTGCTGCCATCTCAAAAACATTTGGACTGCTCCGCTTCCTCCTGAGACT20.10309278350515465No Hit
TCCGAGATTATGCGCCAAATGCTTACTCAAGCTCAAACGGCTGGTCAGTAT20.10309278350515465No Hit
TAAATCGAAGTGGACTGCTGGCGGAAAATGAGAAAATTCGACCTATCCTTG20.10309278350515465No Hit
ACCTTGAATTGCTGGGAACTGTTGGAGTTGGAATATTGCTTTGTATGCAGT20.10309278350515465No Hit
ACACGCAGGACGCTTTTTCACGTTCTGGTTGGTTGTGGCCTGTTGATGCTA20.10309278350515465No Hit
TCATGGTAGAGATTCTCTTGTTGACATTTTAAAAGAGCGTGGATTACTATC20.10309278350515465No Hit
AGCTGGTTCTCACTTCTGTTACTCCAGCTTCTTCGGCACCTGTTTTACAGA20.10309278350515465No Hit
TTTGAGCGTATCGAGGCTCTTAAACCTGCTATTGAGGCTTGTGGCATTTCT20.10309278350515465No Hit
CAGCAAGAGCAGAAGCAATACCGCCAGCAATAGCACCAAACATAAATCACC20.10309278350515465No Hit
AGGCCTCCACTATGAAATCGCGTAGAGGCTTTGCTATTCAGCGTTTGATGA20.10309278350515465No Hit
TGTGTGACTATTGACGTCCTTCCCCGTACGCCGGGCAATAATGTTTATGTT20.10309278350515465No Hit
GCCCCTTACTTGAGGATAAATTATGTCTAATATTCAAACTGGCGCCGAGCG20.10309278350515465No Hit
TATGCTAATTTGCATACTGACCAAGAACGTGATTACTTCATGCAGCGTTAC20.10309278350515465No Hit
TGCCTGACCGTACCGAGGCTAACCCTAATGAGCTTAATCAAGATGATGCTC20.10309278350515465No Hit
GCACTAACCTTGCGAGTCATTTCTTTGATTTGGTCATTGGTAAAATACTGA20.10309278350515465No Hit
GTCGCGTCGTAACCCAGCTTGGTAAGTTGGATTAAGCACTCCGTGGACAGA20.10309278350515465No Hit
AGATGGACGCCGTTGGCGCTCTCCGTCTTTCTCCATTGCGTCGTGGCCTTG20.10309278350515465No Hit
GCAGGACGCTTTTTCACGTTCTGGTTGGTTGTGGCCTGTTGATGCTAAAGG20.10309278350515465No Hit
GGTAATTATACTCATCGCGAATATCCTTAAGAGGGCGTTCAGCAGCCAGCT20.10309278350515465No Hit
CCACATCTATTGACATTATGGGTCTGCAAGCTGCTTATGCTAATTTGCATA20.10309278350515465No Hit
GTTTATGGTGAACAGTGGATTAAGTTCATGAAGGATGGTGTTAATGCCACT20.10309278350515465No Hit
GAGAAGTTAATGGATGAATTGGCACAATGCTACAATGTGCTCCCCCAACTT20.10309278350515465No Hit
CAAAAACATTTGGACTGCTCCGCTTCCTCCTGAGACTGAGCTTTCTCGCCA20.10309278350515465No Hit
ATAGTTTGACGGTTAATGCTGGTAATGGTGGTTTTCTTCATTGCATTCAGA20.10309278350515465No Hit
TGGACTGCTCCGCTTCCTCCTGAGACTGAGCTTTCTCGCCAAATGACGACT20.10309278350515465No Hit
ATCACCTTCTTGAAGGCTTCCCATTCATTCAGGAACCGCCTTCTGGTGATT20.10309278350515465No Hit
ACCGATATTGCTGGCGACCCTGTTTTGTATGGCAACTTGCCGCCGCGTGAA20.10309278350515465No Hit
AGTCGCCGACTGAATGCCAGCAATCTCTTTTTGAGTCTCATTTTGCATCTC20.10309278350515465No Hit
GTTTGCTGATGAACTAAGTCAACCTCAGCACTAACCTTGCGAGTCATTTCT20.10309278350515465No Hit
GCTTCCTCCTGAGACTGAGCTTTCTCGCCAAATGACGACTTCTACCACATC20.10309278350515465No Hit
AAAGCCTCTACGCGATTTCATAGTGGAGGCCTCCAGCAATCTTGAACACTC20.10309278350515465No Hit
CTGGAAACACTGGTCATAATCATGGTGGCGAATAAGTACGCGTTCTTGCAA20.10309278350515465No Hit
GGCAATCTCTTTCTGATTGTCCAGTTGCATTTTAGTAAGCTCTTTTTGATT20.10309278350515465No Hit
AAGCAGAAAACCTACCGCGCTTCGCTTGGTCAACCCCTCAGCGGCAAAAAT20.10309278350515465No Hit
CACACCAGAAGCAGCATCAGTGACGACATTAGAAATATCCTTTGCAGTAGC20.10309278350515465No Hit
AATCTCGGAAACCTGCTGTTGCTTGGAAAGATTGGTGTTTTCCATAATAGA20.10309278350515465No Hit
AGTTGTTGCAGTGGAATAGTCAGGTTAAATTTAATGTGACCGTTTATCGCA20.10309278350515465No Hit
AATGTCTAAAGGTAAAAAACGTTCTGGCGCTCGCCCTGGTCGTCCGCAGCC20.10309278350515465No Hit
TTGAATGTTGACGGGATGAACATAATAAGCAATGACGGCAGCAATAAACTC20.10309278350515465No Hit
CTATAAACATTCTGTGCCGCGTTTCTTTGTTCCTGAGCATGGCACTATGTT20.10309278350515465No Hit
CTCCCAAGCATTAAGCTCAGGAAATGCAGCAGCAAGATAATCACGAGTATC20.10309278350515465No Hit
GGAAACCTGCTGTTGCTTGGAAAGATTGGTGTTTTCCATAATAGACGCAAC20.10309278350515465No Hit
AAAGAGATTCAGTACCTTAACGCTAAAGGTGCTTTGACTTATACCGATATT20.10309278350515465No Hit
TGATTCTCAAATCCGGCGTCAACCATACCAGCAGAGGAAGCATCAGCACCA20.10309278350515465No Hit
GATTAAACTCCTAAGCAGAAAACCTACCGCGCTTCGCTTGGTCAACCCCTC20.10309278350515465No Hit
AAAGCGCCGTGGATGCCTGACCGTACCGAGGCTAACCCTAATGAGCTTAAT20.10309278350515465No Hit
TGCAACGTACCTTCAAGAAGTCCTTTACCAGCTTTAGCCATAGCACCAGAA20.10309278350515465No Hit
GCTTTTCTTGGCACGATTAACCCTGATACCAATAAAATCCCTAAGCATTTG20.10309278350515465No Hit
TATTGACTCTACTGTAGACATTTTTACTTTTTATGTCCCTCATCGTCACGT20.10309278350515465No Hit
CAGCGCCTTCCATGATGAGACAGGCCGTTTGAATGTTGACGGGATGAACAT20.10309278350515465No Hit
TGTTGGTGCTGATATTGCTTTTGATGCCGACCCTAAATTTTTTGCCTGTTT20.10309278350515465No Hit
AGAAACGGCAGAAGTGCCAGCCTGCAACGTACCTTCAAGAAGTCCTTTACC20.10309278350515465No Hit
GAGTATAATTACCCCAAAAAGAAAGGTATTAAGGATGAGTGTTCAAGATTG20.10309278350515465No Hit
AGACTTGCCACCAAGTCCAACCAAATCAAGCAACTTATCAGAAACGGCAGA20.10309278350515465No Hit
ACCAGTCCTTGACGAACGTGCCAAGCATATTAAGCCACTTCTCCTCATCCA20.10309278350515465No Hit
CGCCATGTCTAAATTGTTTGGAGGCGGTCAAAAAGCCGCCTCCGGTGGCAT20.10309278350515465No Hit
CAGGTTGTTTCTGTTGGTGCTGATATTGCTTTTGATGCCGACCCTAAATTT20.10309278350515465No Hit
TTTTAAAGCGCCGTGGATGCCTGACCGTACCGAGGCTAACCCTAATGAGCT20.10309278350515465No Hit
GATTAACCCTGATACCAATAAAATCCCTAAGCATTTGTTTCAGGGTTATTT20.10309278350515465No Hit
CATTTTCATCCCGAAGTTGCGGCTCATTCTGATTCTGAACAGCTTCTTGGG20.10309278350515465No Hit
GCTGAATAGCAAAGCCTCTACGCGATTTCATAGTGGAGGCCTCCAGCAATC20.10309278350515465No Hit
CTGGCGACTCCTTCGAGATGGACGCCGTTGGCGCTCTCCGTCTTTCTCCAT20.10309278350515465No Hit
GAATCAGCGGTATGGCTCTTCTCATATTGGCGCTACTGCAAAGGATATTTC20.10309278350515465No Hit
CGGCACCTGTTTTACAGACACCTAAAGCTACATCGTCAACGTTATATTTTG20.10309278350515465No Hit
ACCGCCCCGAAGGGGACGAAAAATGGTTTTTAGAGAACGAGAAGACGGTTA20.10309278350515465No Hit
TTTTTGCCGCTGAGGGGTTGACCAAGCGAAGCGCGGTAGGTTTTCTGCTTA20.10309278350515465No Hit
TAAGCAATGACGGCAGCAATAAACTCAACAGGAGCAGGAAAGCGAGGGTAT20.10309278350515465No Hit
ACCTGTTTTACAGACACCTAAAGCTACATCGTCAACGTTATATTTTGATAG20.10309278350515465No Hit
TAAGAGGTTTTACCTCCAAATGAAGAAATAACATCATGGTAACGCTGCATG20.10309278350515465No Hit
AAATCCAAAACGGCAGAAGCCTGAATGAGCTTAATAGAGGCCAAAGCGGTC20.10309278350515465No Hit
GAAACGGCAGAAGTGCCAGCCTGCAACGTACCTTCAAGAAGTCCTTTACCA20.10309278350515465No Hit
CATGCGGCATACGCTCGGCGCCAGTTTGAATATTAGACATAATTTATCCTC20.10309278350515465No Hit
GCAGGCTGGCACTTCTGCCGTTTCTGATAAGTTGCTTGATTTGGTTGGACT20.10309278350515465No Hit
GCGAATATCCTTAAGAGGGCGTTCAGCAGCCAGCTTGCGGCAAAACTGCGT20.10309278350515465No Hit
CGTACGGATTGTTCAGTAACTTGACTCATGATTTCTTACCTATTAGTGGTT20.10309278350515465No Hit
CAAAGTCAAAATAATCAGCGTGACATTCAGAAGGGTAATAAGAACGAACCA20.10309278350515465No Hit
GATTGGCGTATCCAACCTGCAGAGTTTTATCGCTTCCATGACGCAGAAGTT20.10309278350515465No Hit
AGTTTGAATATTAGACATAATTTATCCTCAAGTAAGGGGCCGAAGCCCCTG20.10309278350515465No Hit
CTGGTTCTCACTTCTGTTACTCCAGCTTCTTCGGCACCTGTTTTACAGACA20.10309278350515465No Hit
AGAATCTCTACCATGAACAAAATGTGACTCATATCTAAACCAGTCCTTGAC20.10309278350515465No Hit
CAAATGACGACTTCTACCACATCTATTGACATTATGGGTCTGCAAGCTGCT20.10309278350515465No Hit
AGATTTGTCGTCACAGGTTGCGCCGCCAAAACGTCGGCTACAGTAACTTTT20.10309278350515465No Hit
CACGAGTATCCTTTCCTTTATCAGCGGCAGACTTGCCACCAAGTCCAACCA20.10309278350515465No Hit
GTGGCCTTGCTATTGACTCTACTGTAGACATTTTTACTTTTTATGTCCCTC20.10309278350515465No Hit
GTAGGAAGTGTCCGCATAAAGTGCACCGCATGGAAATGAAGACGGCCATTA20.10309278350515465No Hit
CCTACAGTATTGTTATCGGTAGCAAGCACATCACCTTGAATGCCACCGGAG20.10309278350515465No Hit
ATGACCTTTCCCATCTTGGCTTCCTTGCTGGTCAGATTGGTCGTCTTATTA20.10309278350515465No Hit
TTTCTTTGATTTGGTCATTGGTAAAATACTGACCAGCCGTTTGAGCTTGAG20.10309278350515465No Hit
CAAAGTAAGAGCTTCTCGAGCTGCGCAAGGATAGGTCGAATTTTCTCATTT20.10309278350515465No Hit
CTAGACCTTTAGCAGCAAGGTCCATATCTGACTTTTTGTTAACGTATTTAG20.10309278350515465No Hit
ATCGTTATGCGCCTTCGTATGTTTCTCCTGCTTATCACCTTCTTGAAGGCT20.10309278350515465No Hit
CTCGCCATAATTCAAACTTTTTTTCTGATAAGCTGGTTCTCACTTCTGTTA20.10309278350515465No Hit
TTATCGAAGCGCGCATAAATTTGAGCAGATTTGTCGTCACAGGTTGCGCCG20.10309278350515465No Hit
CATTAGAGCCTTGAATGGCAGATTTAATACCAGCATCACCCATGCCTACAG20.10309278350515465No Hit
TGGCGGCGATTGCGTACCCGACGACCAAAATTAGGGTCAACGCTACCTGTA20.10309278350515465No Hit
TAATATTCAAACTGGCGCCGAGCGTATGCCGCATGACCTTTCCCATCTTGG20.10309278350515465No Hit
AAGCAACTTATCAGAAACGGCAGAAGTGCCAGCCTGCAACGTACCTTCAAG20.10309278350515465No Hit
TGGATAACCGCATCAAGCTCTTGGAAGAGATTCTGTCTTTTCGTATGCAGG20.10309278350515465No Hit
AATAACCGTCAGGATTGACACCCTCCCAATTGTATGTTTTCATGCCTCCAA20.10309278350515465No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)