FastQCFastQC Report
Fri 23 Dec 2016
H5WJNBCXY_l01n02_gfp1_18hpf_12_8.35200000074845.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5WJNBCXY_l01n02_gfp1_18hpf_12_8.35200000074845.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22036141
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGACG45600.019.2235545
CTGACGC27250.014.04778245
TATACTG73100.011.7233455
ATCTGAC105850.010.44278945
TATACAC437900.010.21079945
TATACCG13450.010.0339735
CTTATAC670700.09.66222345
TTATACA584250.09.587666545
TCTATAC52650.09.5269833
GTGTAAG47000.09.3964011
TACACCG11500.09.38830955
TCTTATA892150.09.38640145
CTATACT53300.09.2841354
CTAGACG7600.08.8787874
CCTACAC41150.08.8551133
GTATAAG54400.08.7809261
GTGTTAG51950.08.76131251
ATACACA380500.08.75680345
GTATAGA56700.08.6631691
GTATTAG59900.08.6141411
Produced by FastQC (version 0.11.2)