FastQCFastQC Report
Tue 11 Apr 2017
H5WJNBCXY_l02n02_handr_mekmut1_18hpf_12_13.352000000747d9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5WJNBCXY_l02n02_handr_mekmut1_18hpf_12_13.352000000747d9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences102893641
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG407750.010.6267595
AACCGCG158600.09.911117
TATACCG240200.09.8123765
TACACTG397950.09.7899845
TATACAC1832350.09.53562845
GTATAAG480200.09.3680981
CGGGACT192700.09.33584510
GGACGAC140500.09.2672437
CTGGACG129750.09.1520585
ACCGCGG149000.09.0253338
TCGGGAC199900.09.0108989
CTAATAC370300.08.9576163
CTTATAC2674450.08.89068645
ATACACA1402650.08.87399645
GTCTTAG124650.08.8098271
GCGGTAG132500.08.79142511
CGTCCAG109450.08.75264310
TAGACCG167750.08.744485
TTATACT628450.08.7417544
GTGTAGG259650.08.7365581
Produced by FastQC (version 0.11.2)