FastQCFastQC Report
Tue 11 Apr 2017
H5WJNBCXY_l02n02_handr_dnfgfr_18hpf_12_9_2000_cells.35200000074852.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5WJNBCXY_l02n02_handr_dnfgfr_18hpf_12_9_2000_cells.35200000074852.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15886697
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG35450.011.8862015
TACACTG62700.011.5719375
TAGACCG31750.011.5680895
TATACTG68700.011.2500675
CTTATAC331700.010.49494645
GTCTAGT19850.010.3379141
TAGACGG12100.010.2422425
CTATACT59750.010.2205384
TAGACAG28700.010.2065545
CTGAGCG24450.010.1172249
ACTGTGC33350.09.9796068
GTCTAGG7700.09.9572691
CTAATAC46000.09.8465953
TGAGCGA26950.09.84609210
CTACACT50350.09.7118644
TCTATAC49050.09.6937383
GTATTAG51000.09.6833571
GACCTAG21700.09.63748910
ACCGCTC17050.09.628228
AGACCGT38100.09.6207766
Produced by FastQC (version 0.11.2)