FastQCFastQC Report
Tue 11 Apr 2017
H5WJNBCXY_l02n02_gfp_10hpf_june_2.3520000007487b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5WJNBCXY_l02n02_gfp_10hpf_june_2.3520000007487b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1093
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAATAGATGTGGTAGAAGTCGTCATTTGGCGAGAAAGCTCAGTCTCAGGAG20.18298261665141813No Hit
AAACTTTTTTTCTGATAAGCTGGTTCTCACTTCTGTTACTCCAGCTTCTTC20.18298261665141813No Hit
AGCGACGAGCACGAGAGCGGTCAGTAGCAATCCAAACTTTGTTACTCGTCA20.18298261665141813No Hit
GTTAGCATGTATATAACGTATTATCCATCTTCTATTTACATCTCGTATAAT20.18298261665141813No Hit
GGTTTTCTTCATTGCATTCAGATGGATACATCTGTCAACGCCGCTAATCAG20.18298261665141813No Hit
GTTGTCACTTTAAGGTTTAGGTTCCCATTAGTTTCTTTTTTTCACTTATGT20.18298261665141813No Hit
TAACTGTTCATTCCTTATATTTAATCTTATTCTCATTAATTGTAATAGTAA20.18298261665141813No Hit
AAAAATTACGTGCAGAAGGAGTGATGTAATGTCTAAAGGTAAAAAACGTTC20.18298261665141813No Hit
AACAGTCGGGAGAGGAGTGGCATTAACACCATCCTTCATGAACTTAATCCA20.18298261665141813No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)