FastQCFastQC Report
Tue 11 Apr 2017
H5WJNBCXY_l02n01_handr_dnfgfr_18hpf_10_6.351000000747f6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5WJNBCXY_l02n01_handr_dnfgfr_18hpf_10_6.351000000747f6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10189587
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCGAG13250.015.7938245
TAGACCG19250.013.9080425
TATACAC241500.013.78069745
TCTCCGA25250.012.56544445
TTATACA346600.012.4844945
CCGTGCG11100.012.1620819
ATACACA166350.011.84953845
ACCGTGC20200.011.361318
CTTATAC431100.011.33709945
GTCTAGC17250.011.1210491
TACACTG34950.011.0077535
AGACCGT21600.010.6243556
GTCCTAC12200.010.54462051
GTCTTAC18000.010.5322891
TATACCG19700.010.5068225
TCTTATA557600.010.47212345
CTAGACT11050.010.3830184
TACACAT107750.010.35820645
TATCCCG12000.010.1242385
TAGACTG17400.09.9561465
Produced by FastQC (version 0.11.2)