FastQCFastQC Report
Tue 11 Apr 2017
H5WJNBCXY_l02n01_gfp2_18hpf_12_08.3510000007483b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5WJNBCXY_l02n01_gfp2_18hpf_12_08.3510000007483b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48200324
Sequences flagged as poor quality0
Sequence length51
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCACGAG67950.021.75188826
ACCGAGG67850.021.55475833
CCGAGGC68750.021.2398634
TCCGAGC69900.021.14509819
CCGAGCC70100.020.98847220
AGCCCAC70350.020.97784223
CCCACGA71550.020.75178125
GCCCACG71350.020.58923724
AGACCGA71050.020.42475331
CGAGGCT74350.019.57956135
CGAGCCC75600.019.43177421
CTCCGAG75350.019.31714818
ACGAGAC78000.019.23764228
CGAGACC78600.019.03354629
CACGAGA78850.018.77348127
AGGCTGA76950.018.74239237
GAGCCCA79350.018.68355822
GAGACCG80050.018.12840530
TGATCTC73950.017.73921241
GCTGATC74450.017.67790239
Produced by FastQC (version 0.11.2)