FastQCFastQC Report
Tue 11 Apr 2017
H5WJNBCXY_l01n02_handr_mekmut2_18hpf_12_13.35200000074888.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5WJNBCXY_l01n02_handr_mekmut2_18hpf_12_13.35200000074888.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32204909
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG135650.09.9819515
TACACTG128500.09.2595815
TATACCG73600.09.1375995
CGGGACT48400.09.01440410
GTATAGA115500.08.9740461
TAGACCG58500.08.9201225
CTCTTAT978500.08.89678445
TATACAC498600.08.85488745
TATACAG159600.08.8222675
CTATACT118950.08.7928554
CTTATAC693450.08.7808745
AACCGCG50850.08.7136177
TCTATAC111700.08.6991073
GTATAAG153700.08.6348311
CTAATAC107150.08.58569053
GTATAGG97850.08.4972421
GTCTAGT42450.08.4397951
TTATACT189350.08.398384
GTCTTAG33600.08.3826851
TCTCTTA1191750.08.31793745
Produced by FastQC (version 0.11.2)