FastQCFastQC Report
Tue 11 Apr 2017
H5WJNBCXY_l01n02_handr_lacz_18hpf_12_08.35200000074801.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5WJNBCXY_l01n02_handr_lacz_18hpf_12_08.35200000074801.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5430215
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG12550.012.0083645
GTCTAGT7900.011.408461
TACACTG21050.011.2199265
TAGACAG11250.011.1966545
GTCCTAT9550.010.8529691
TAGGACC8100.010.8300974
CTAGACT6900.010.4316664
AGTACCG6300.010.354055
CTTATAC137550.010.17762345
CTAATAC17250.09.9100833
GTACTAG9100.09.9040471
ACTAGAC8000.09.840813
TATACTG22400.09.840815
CCGTGCG5550.09.7256579
GTAGGAC7200.09.6846073
TCCTAGG5600.09.6398882
TATACAC93200.09.62493345
CTAGCAC7750.09.5777843
ACCGTCC5701.8189894E-129.469728
ATACACA73450.09.46814845
Produced by FastQC (version 0.11.2)