FastQCFastQC Report
Tue 11 Apr 2017
H5WJNBCXY_l01n02_handr_lacz_18hpf_10_6.3520000007486e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5WJNBCXY_l01n02_handr_lacz_18hpf_10_6.3520000007486e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10024291
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTGCG11050.012.0081129
TAGACCG19700.011.18942455
TATACAC181600.011.15169145
ACCGTGC14900.010.8675388
AGACCGT21650.010.2848266
CCGTCCC8450.010.1137429
TACACTG39650.010.0410235
ACCGTCC10100.010.0201948
CTTATAC282800.09.93256345
ATACACA136550.09.55542145
GTCTTAC17100.09.4871881
CTACACT32750.09.4779784
TCTTATA375650.09.38307745
GACCGTG13500.09.3298347
GTCGTCC14000.09.317198
TTATACA259950.09.23708945
GTATTAG42550.09.16111
AGTACCG14100.09.0929265
AACCGCG20300.09.0852637
TAGACAG17350.09.0749965
Produced by FastQC (version 0.11.2)