FastQCFastQC Report
Tue 11 Apr 2017
H5WJNBCXY_l01n02_handr_dnfgfr_18hpf_12_9_600cells.3520000007482b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5WJNBCXY_l01n02_handr_dnfgfr_18hpf_12_9_600cells.3520000007482b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7581565
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACCG13650.012.8533355
TACACTG29900.012.2622555
TATACCG16400.012.0695955
TATACTG31200.011.8234215
GTCTAGT9800.011.7266231
ACCGCTC8350.011.3128198
ACTACAC18200.011.1231533
GTATTAG31700.011.08904651
GTATAAG34450.011.0541741
ACTGACC14850.010.9047088
AGACCGT15550.010.5592256
CTACACT25350.010.5590034
ATCCTAG8750.010.5585591
ACAGTGC15900.010.4674918
CTATACT24950.010.367674
CTTATAC153150.010.30798745
CTGACCA15000.010.1959039
GTACCGT10400.010.1649236
GTATACG16050.010.1084911
GTGTAGG21000.010.0864291
Produced by FastQC (version 0.11.2)