FastQCFastQC Report
Tue 11 Apr 2017
H5WJNBCXY_l01n01_handr_mekmut2_18hpf_12_13.3510000007488b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5WJNBCXY_l01n01_handr_mekmut2_18hpf_12_13.3510000007488b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32204909
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG77050.09.9569925
TACACTG123350.09.885685
TATACTG134150.09.8612765
TAGACCG59100.09.8595875
ATCTCCG71550.08.96142245
CTCTTAT991150.08.8706845
GTGTAGG83550.08.7837011
TTATACT182300.08.7746144
ATACCGT77900.08.7508916
CTTATAC701000.08.71351845
GTCTTAG36600.08.6991871
TATACAC515100.08.65672945
CTATACT122000.08.556644
AGACCGT65850.08.5414456
GTATAGA118100.08.5275761
TATACAG158500.08.4740595
TAGGTCG55450.08.4393345
GTATAGG98850.08.4064341
CGTCCAG32400.08.40209210
AACCGCG48900.08.3275417
Produced by FastQC (version 0.11.2)