FastQCFastQC Report
Tue 11 Apr 2017
H5WJNBCXY_l01n01_handr_lacz_18hpf_12_08.35100000074804.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5WJNBCXY_l01n01_handr_lacz_18hpf_12_08.35100000074804.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5430215
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTGC6400.011.2490448
CTAGCAC7100.010.4566663
TACACTG22150.010.3602935
ACGGTCC3704.1836756E-910.336968
CCGTCCC3951.1241355E-910.2522949
TACACAG15150.010.24665455
TATACCG11900.010.2092175
CTTATAC141100.010.12484545
CTGAGCG7800.010.0952969
CTATACT19250.09.9342214
CCGTGCG5251.8189894E-129.8563059
CTGTGCG6650.09.8111979
TATACTG23000.09.7817785
TAGGTCG9200.09.7817775
ACTGAGC13650.09.7244498
TAGACCG10000.09.6741785
ATACACA72350.09.67082545
TACACCG9550.09.6588665
CTCGGGG4458.62201E-109.60574930
TCTATAC18600.09.5554643
Produced by FastQC (version 0.11.2)