FastQCFastQC Report
Tue 11 Apr 2017
H5WJNBCXY_l01n01_handr_lacz_18hpf_10_6.35100000074861.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5WJNBCXY_l01n01_handr_lacz_18hpf_10_6.35100000074861.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10024291
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCCG6450.012.5571229
TAGACCG18050.011.965795
CTCCGAG13150.011.46277845
ACCGTCC10350.011.0860588
TACACTG38500.010.8692495
ACCGTGC16800.010.8473358
GAGTAGG13950.010.8449021
TATACTG38450.010.8248715
TATACAC187100.010.79800145
CCGTGCG10800.010.624149
CTTATAC284200.09.966495545
CTATACT35350.09.864784
ATACACA140000.09.49730345
ACCGCGG15400.09.4959838
TAGGACT8800.09.4594144
TCTTATA376200.09.43091345
CTACACT32050.09.4063664
AGTACCG12950.09.3814795
ATCTCCG27900.09.3539445
TTATACA261300.09.28154345
Produced by FastQC (version 0.11.2)