FastQCFastQC Report
Tue 11 Apr 2017
H5WJNBCXY_l01n01_handr_dnfgfr_18hpf_12_9_600cells.3510000007482e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5WJNBCXY_l01n01_handr_dnfgfr_18hpf_12_9_600cells.3510000007482e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7581565
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAGT8900.012.6839721
TAGACCG15000.012.4493635
TACACTG29500.012.1264985
TATACTG30300.011.8063275
ACCGCTC9100.011.3730468
TACACCG12950.011.11912255
AGTACCG7200.010.9369415
CCGTCCG4750.010.8941799
AACCGCG10200.010.5876947
ACCGTCC7950.010.4711638
CTTATAC153100.010.448173545
TACCGTC8250.010.3631077
CTATACT26350.010.2460194
TTATACT41950.010.243684
ACTGTGC16100.010.2013418
GTATAAG32650.010.1650481
TATACCG16250.010.1071765
CCGCTCG8300.010.0296089
TCTATAC25600.09.9306743
CTAGACG5450.09.9076194
Produced by FastQC (version 0.11.2)